Electrical Power Safety
1. Limit the use of high power devices around water.
2. Never use frayed or cut wires when plugging in a device.
3. Never overload the outlet and use a surge protector.
4. All electrical devices should have an emergency cut off switch or ‘kill’ switch’.
5. Shut off and unplug all devices when not in use or when you leave the room
1. Limit the use of high power devices around water.
2. Never use frayed or cut wires when plugging in a device.
3. Never overload the outlet and use a surge protector.
4. All electrical devices should have an emergency cut off switch or ‘kill’ switch’.
5. Shut off and unplug all devices when not in use or when you leave the room
unattended.
Power Tools
1. Wear goggles or safety glasses
2. Do not work alone in the lab.
3. Dry wet hands and clothing before working with electricity. Mop up all water spilled
1. Wear goggles or safety glasses
2. Do not work alone in the lab.
3. Dry wet hands and clothing before working with electricity. Mop up all water spilled
on the floor.
4. Keep the lab clean.
5. Clamp down any objects that will be used.
6. Know operation of all switches and controls before using a power tool.
7. Keep hands, fingers, feet, and all objects not being used way from moving parts.
8. Use a vacuum system to keep debris away from cutting area.
4. Keep the lab clean.
5. Clamp down any objects that will be used.
6. Know operation of all switches and controls before using a power tool.
7. Keep hands, fingers, feet, and all objects not being used way from moving parts.
8. Use a vacuum system to keep debris away from cutting area.
Sharp Object Safety
1. Use caution when using a sharp object.
2. Restrict the use of "sharps" to a minimum.
3. Do not use bent or broken needles, knifes, or razor blades.
4. Cover or cap needle after every use.
5. Discard used syringes, needles, or scalpels into an approved sharps container.
6. Bring all needles in a sharps container to a local fire station for disposal.
1. Use caution when using a sharp object.
2. Restrict the use of "sharps" to a minimum.
3. Do not use bent or broken needles, knifes, or razor blades.
4. Cover or cap needle after every use.
5. Discard used syringes, needles, or scalpels into an approved sharps container.
6. Bring all needles in a sharps container to a local fire station for disposal.
Building the Environmental Collection Device1. Take three 1 inch diameter PVC pipes, for a total of nine PVC pipes for each of the
habitats.
2. Measure out each PVC pipe to 6 inches.
3. Cut the PVC pipes with a jig saw and cap one end of each pipe with PVC glue.
4. To hold the pipes together glue PVC glue between them.
5. Get one kayak strap and wind it around the nine PVC pipes so the pipes are securely
tied.
6. Repeat steps 1-5 with 1.5 inch diameter PVC pipes.
7. Repeat steps 1-5 with 2 inch diameter PVC pipes.
Choosing a Location
1. Look for a location that has relatively the same amount of sea grass beds and terrain.
Building the Holding Bin
1. Take a tub of any size comfortable, make sure it is bigger than two gallons, and drill 6
holes a ½ inch from the top big enough for zip ties to fit through.
2. Drill another set of holes 2.5 inches beneath your first set.
3. Take a pool noodle and wrap it around the top of the tube.
4. Insert one end of the zip tie into the top hole of the tube and wrap it around the pool
noodle into the bottom hole.
5. Close it.
6. Repeat the same for the rest of the tube.
Collecting the Environmental Collection Device
1. Disturbing the water as little as possible, reach out to the handle and quickly pull up
with the cap end down.
2. Scoop some water out of the lagoon at the location and pour it into the holding bin.
3. Take the contents of the Environmental Collection Device and dump the contents into
the holding bins.
4. Replace the Environmental Collection Device into the same location you had it in.
5. Do your observations immediately with little contact to the organisms. Do NOT take
them with you.
6. Repeat 1-6 for the other habitats.
Seining
1. Fill the holding bin partially with water.
2. Take the seine and the holding bin and wade out into the water, with another person.
3. Place the weighted end of the net into the water. The net has to at least be
three-quarters of a way in the water and the bottom touching the ground.
4. Have both people pull on either end so the seine is completely stretched out.
5. Both people walk slowly parallel to the shore for twenty steps.
6. Pull the net up out of the water weighted end first.
7. Quickly transfer all organisms into the bucket.
Observations of the Collection Device and Seining
1. Bring an identification book with you to each habitat.
2. For each organism you collect size the organism and identify it.
3. Record at what location you got this organism, it’s size, what it is, and what Device it
was found in.
4. Do the same for all organisms.
T-Test
1. Compare the seines first.
2. Use Road and Sand, find the means for both.
3. Take the means and subtract Sand from Road.
4. Use excel to find the variance for both.
4. Use excel to find the variance for both.
5. Divide the variance by the number of trials for both.
6. Add the numbers from Step 5 together.
7. Square root the numbers from Step 6.
8. Divide Step 3 from Step 7.
9. The alpha will be 0.05.
10.The Degrees of Freedom will be the trials from Sand added to the trials from Road
minus 2.
11.Use the Critical Value chart and locate the column for 0.05 one-tail, and the row for
the Degrees of Freedom, and this number will be the Critical Value.
12.Compare the T-Test value from Step 8 with the Critical Value from the chart.
